Microscopy simple staining

C Bacillus cereus a gram-positive rod found in the soil. The differential stain technique distinguishes two kinds of organisms.

simple staining procedure

Magnification alone is not the only aim of a microscope. Stains serve several purposes: Stains differentiate microorganisms from their surrounding environment They allow detailed observation of microbial structures at high magnification Certain staining protocols can help to differentiate between different types of microorganisms.

This can be prevented by never viewing a specimen with the 40X objective after adding immersion oil to a slide.

simple staining observation result and conclusion

In order to obtain reliable results it is important to take the following precautions: The cultures to be stained should be young - incubated in broth or on a solid medium until growth is just visible no more than 12 to 18 hours old if possible. For example, one group, the gram-positive bacteria, include the causative agents of the diseases diphtheria, anthrax, tetanus, scarlet fever, and certain forms of pneumonia and tonsillitis.

Simple staining definition

To intensify the stain, a chemical called mordant can be added. The compound microscope used in microbiology is a precision instrument; its mechanical parts, such as the calibrated mechanical stage and the adjustment knobs, are easily damaged, and all lenses, particularly the oil immersion objective, are delicate and expensive. Place the coverslip directly onto the drop on the slide in such a manner that some air bubbles are trapped. Move the microscope condenser by means of the condenser adjustment knob until the top of the condenser is almost at the highest position. Capsules appear clear or as halos if present. Microscopes also have important roles in genetics, cell structure, biochemistry and many other scientific disiplines. Place the three fixed smears on a staining loop or rack over a sink. Demonstrate the Parfocal Property of the Microscope with the Oil Immersion Objective Obtain one of the prepared tissue or bacterial slides. Stains are often diluted in liquid before applying to the slide.

Demonstrate the Parfocal Property of the Microscope with the Oil Immersion Objective Obtain one of the prepared tissue or bacterial slides. Once again, position an interesting structure in the center of the field, rotate the high dry objective half way out of the way - enough so that you can deposit a small drop of oil onto the center of the lighted specimen.

simple staining ppt

Figure 2.

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Simple Staining: Principle, Procedure and Results